인테그린 α2와 상피성장인자수용체 차단항체의 저해작용을 통한 구강편평상피암 세포의 선택적 제거
Induction of Selective Cell Death of Oral Squamous Carcinoma Cells by Integrin α2 Antibody and EGFR Antibody
최연식, 황대석, 신상훈, 김욱규, 김규천, 윤식, 김철훈, 전영찬, 변준호,
소속 상세정보
최연식 ( Choi Yeon-Sik ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
황대석 ( Hwang Dae-Seok ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
신상훈 ( Shin Sang-Hun ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치의학전문대학원 구강해부학교실
윤식 ( Yoon Sik ) - 부산대학교 의학전문대학원 해부학교실
김철훈 ( Kim Chul-Hoon ) - 동아대학교 의과대학 구강악안면외과학교실
전영찬 ( Jeon Yeong-Chan ) - 부산대학교 치의학전문대학원 보철학교실
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 구강악안면외과학교실
KMID : 1036320130350030143
Abstract
Purpose: This study was to find efficacy of integrin alpha2 (${\alpha}_2$ 수식 이미지) and epidermal growth factor receptor (EGFR) as tumor marker of oral squamous cell carcinoma (SCC) and clarify the selective cell death effect of anti-integrin ${\alpha}_2$ 수식 이미지 and anti-EGFR on SCC cells, additionally testify conjugated gold nanoparticles (GNP) with air plasma for selective cell death of oral SCC.
Methods: Expression of integrin ${\alpha}_2$ 수식 이미지, EGFR on human SCC cells (SCC25) were examined by western blot. SCC25 cells were treated with anti-integrin ${\alpha}_2$ 수식 이미지, anti-EGFR and analysed by Hemacolor staining, immunoflorescence staining, FACS flow cytometry. Conjugated GNP with integrin ${\alpha}_2$ 수식 이미지, EGFR antibody were treated by air plasma on SCC cells.
Results: Integrin ${\alpha}_2$ 수식 이미지 and EGFR were over-expressed on SCC25 cells than normal lung WI-38 cells. The cell viability rate of SCC25 cells treated with anti-integrin ${\alpha}_2$ 수식 이미지, anti-EGFR was lower than WI-38 cells. The concentration changes of nucleus, releasing cytochrome c and apoptosis inducing factor (AIF) from mitochondria to cytosol were observed. The changes of proteins related with apoptosis were observed. Increase of bax, bcl-xL, activation of caspase-3, -7, -9, and fragmentation of PARP, DFF45 and decrease of lamin A/C in SCC25 cells were observed. In FACS, increase of sub-$G_1$ 수식 이미지 and S phase was observed. Cell cycle related proteins, Such as cyclin D1, cyclin dependent kinase (CDK) 4, cyclin A, cyclin E, CDK 2, p27 were decreased. After SCC25 cells treated with conjugatged GNP-Integrin ${\alpha}_2$ 수식 이미지, GNP-EGFR, additionally air plasma, the cell death rate was significantly increased.
Conclusion: Integrin ${\alpha}_2$ 수식 이미지, EGFR were over-expressed in oral SCC cells. Anti-integrin ${\alpha}_2$ 수식 이미지, anti-EGFR in SCC25 cells induced apoptosis selectively. When GNP-anti integrin ${\alpha}_2$ 수식 이미지, GNP-anti EGFR were treated with air plasma on SCC25 cells, cancer cells were died more selectively. GNP-anti integrin ${\alpha}_2$ 수식 이미지, GNP-anti EGFR with air plasma could be treatment choice of oral SCC.
키워드
OSCC;Integrin alpha2;EGFR;Gold nanoparticles;Cell death
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